Absence of Bovine Leukosis Virus in Semen of Seropositive Bulls
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چکیده
منابع مشابه
Bovine leukaemia virus and enzootic bovine leukosis.
Infection of bovines with bovine leukaemia virus (BLV) manifests itself in either of two ways: 30-70% of carriers develop persistent lymphocytosis (PL), with the viral genome integrated at a large number of different sites in the DNA of the affected B-lymphocytes, without causing any chromosomal abnormalities. Only 0,1-10% of carriers develop lymphoid tumours, which also consist of B-lymphocyte...
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A rapid and sensitive reverse transcription polymerase chain reaction (RT-PCR) and nested-PCR were used to detect bovine viral diarrhea virus 1 (BVDV-1) in bull semen. Selected primers could amplify a part of the 5´UTR of the BVDV genome. A 294 bp DNA fragment was amplified and specificity of the results was confirmed by direct sequencing of the PCR product. Prior to RNA extraction, the seminal...
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To study Schmallenberg virus (SBV) excretion in bovine semen after experimental infection, two bulls were inoculated subcutaneously with a SBV isolate (1 ml Vero cell culture 106 TCID50). After inoculation (at day 0), semen was collected daily from both animals for 21 days and samples were tested for SBV by qRT-PCR assay. At 24 days post-inoculation both animals were subjected to necropsy and t...
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1. Nasci RS, Brault AC, Lambert AJ, Savage HM. Novel bunyavirus in domestic and captive farmed animals, Minnesota, USA. Emerg Infect Dis. 2014 Feb;20:337. 2. Xing Z, Schefers J, Schwabenlander M, Jiao Y, Liang M, Qi X, et al. Novel bunyavirus in domestic and captive farmed animals, Minnesota, USA. Emerg Infect Dis. 2013;19:1487–9. http://dx.doi.org/ 10.3201/eid1909.130165 3. Matsuno K, Weisend ...
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ژورنال
عنوان ژورنال: Journal of Veterinary Diagnostic Investigation
سال: 2002
ISSN: 1040-6387,1943-4936
DOI: 10.1177/104063870201400507